Cell density impacts onCandida glabratasurvival in hypo-osmotic stress

hypo-osmotic stress in mammalian cells

Osmoregulation is important to living organisms for survival in responding to environmental changes ofwater and ionic strength. We demonstrated here for the first time that exposure of HeLa cells to a hypotonic medium (30% growth medium and 700% water) prominently induced the binding activity of the heat shock transcription factor (HSF). Pretreatment of cells with cycloheximide did not inhibit ...

Hypo-osmotic urine in Nereis diversicolor.

Although Beadle a9 early as 1937 suggested that the urine of Nereis diversicolor O. F. Miiller might be of lower concentration than the body fluids when this worm is hyperregulating in low salinities, there has been no direct measurement of urine concentration to support this reasonable idea. Jorgensen & Dales (1957) studied osmotic water uptake and loss in N. diversicolor and calculated that t...

Establishment of an optimal hypo-osmotic swelling test by examining single spermatozoa in four different hypo-osmotic solutions.

In order to find an optimal hypo-osmotic swelling test (HOST) and to identify viable sperm cells from patients with asthenozoospermia for intracytoplasmic sperm injection (ICSI), we tested single motile and non-motile spermatozoa in four hypo-osmotic solutions by micromanipulation. The four solutions were: A, H2O; B, 50 mOsm NaCl; C, 150 mOsm NaCl and D, 150 mOsm sodium citrate and fructose. Eo...

RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli.

Hyper-osmotic stress strongly induces expression of the Escherichia coli proU operon encoding a high affinity uptake system for the osmoprotectants glycine betaine and proline betaine. Osmoregulation of proU takes place at the transcriptional level by upregulation of the promoter at high osmolarity and repression of transcription by the nucleoid-associated protein H-NS at low osmolarity. In the...

Integrin α1β1 Is Necessary For Primary Cilia Shortening In Response To Hypo- osmotic Stress